Deciphering High-Resolution 3D Chromatin Organization <em>via</em> Capture Hi-C

The spatial organization of the genome contributes to its function and regulation in many contexts, including transcription, replication, recombination, repair. Understanding exact causality between topology is therefore crucial increasingly subject intensive research. Chromosome conformation capture technologies (3C) allow inferring 3D structure chromatin by measuring frequency interactions any region genome. Here we describe a fast simple protocol perform Capture Hi-C, 3C-based target enrichment method that characterizes allele-specific megabased-sized genomic targets at high-resolution. In regions are captured an array biotinylated probes before downstream high-throughput sequencing. Thus, higher resolution allele-specificity achieved while improving time-effectiveness affordability technology. To demonstrate strengths, Hi-C was applied mouse X-inactivation center (Xic), master regulatory locus X-chromosome inactivation (XCI).